Fig. 2 | Cellular & Molecular Immunology

Fig. 2

From: Uncovering two neutrophil-committed progenitors that immediately precede promyelocytes during human neutropoiesis

Fig. 2The alternative text for this image may have been generated using AI.

Phenotypic comparison of ProNeus with NCP5s, NCP6s, eNePs, and PMs w/o eNePs by flow cytometry. A Flow cytometry gating strategy to identify the ProNeus in SSChiLin-CD45+ cells in BM-LDCs (panel II, green gate) according to their CD66b+CD49d+CD11b- phenotype. B Plots showing how ProNeus (green dots as defined in A) overlay PMs w/o eNePs (yellow gate), eNePs (blue gate), NCP5s (light green gate) and NCP6s (red gate), as defined by the gating strategy illustrated in Fig. 1. C Contour plot overlays highlighting the differences among NCP5s, NCP6s, eNePs, PM w/o eNePs and ProNeus in terms of the SSC-A parameter and CD66b expression. The data are representative of 1 of 4 independent experiments, with similar results

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