Fig. 3

Differentiation of NCP5s and NCP6s into CD66⁺ cells. A Representative flow cytometry gating strategy used to analyze CD66b⁺ cells derived from NCP5s and NCP6s cultured with SFGc for 5 days (n = 10). Plots showing the identification of basophils (bordeaux gate), monocytes (light blue gate), eosinophils (orange gate) and undifferentiated cells (gray gate), as well as PMs (beige gate), MYs (pink gate), MMs (light red gate), BCs (red gate) and SNs (dark red gate) within the CD66b⁺ cells (green gate). B Bar graphs showing the percentages of CD66b⁺ cells (green contour, mean ± s.e.m. refers to total CD45⁺ cells, n = 10), eosinophils (orange contour), basophils (bordeaux contour), monocytes (light blue contour) and undifferentiated cells (gray contour) derived from NCP5s and NCP6s cultured for 5 days with SFGc. C Bar graphs showing the percentages of CD66b⁺PMs (beige contour), MYs (pink contour), MMs (light red contour), BCs (red contour) and SNs (dark red contour) derived from NCP3s, NCP4s, NCP5s and NCP6s cultured for 5 days with SFGc (mean ± s.e.m., n = 5 for NCP3s and NCP4s; n = 10 for NCP5s and NCP6s). D Bar graph representing the generation of SNs (alias CD10⁺ cells) from NCP3s, NCP4s, NCP5s and NCP6s treated with SFGc for 5 days (mean ± s.e.m., n = 5 for NCP3s and NCP4s; n = 10 for NCP5s and NCP6s). E Bar graph displaying the fold expansion of purified NCP3s, NCP4s, NCP5s and NCP6s treated with SFGc for 5 days (mean ± s.e.m., n = 5 for NCP3s and NCP4s; n = 10 for NCP5s and NCP6s). D, E Statistical analysis was performed via one-way ANOVA and Tukey’s post hoc test. * = p < 0.05, ** = p < 0.01, *** = p < 0.001