Fig. 5
Transcriptional and immunocytochemical analyses of the expression profiles of representative azurophilic, specific, and gelatinase granule proteins in NCP5s and NCP6s. A Box plots showing the distribution of mRNA expression levels [log2(FPKM + 1)] of genes associated with AG, SG and GG. The box plot shows the median with the lower and upper quartiles representing the 25th to 75th percentile range and whiskers extending to the 1.5 × interquartile range (IQR). LOESS fitting of the data with a relative confidence interval is represented by a blue line with a shadow area. B Heatmaps showing the expression levels of selected genes encoding typical azurophilic, specific, and gelatinase granule proteins. The median gene expression levels of the biological replicates (n = 3--6) were calculated, and the data were represented as z scores. C, D Expression of antigenic elastase (ELANE), α-defensins (DEFAs) and arginase-1 (ARG1) by immunocytochemical staining of NCP5s, NCP6s and PMs. C Four representative stained cells for each cell population are shown. Original magnification, ×600. D Stacked bar graph displaying the percentage of cells showing negative (white), weakly positive (green), or strongly positive (blue) immunocytochemical staining for ELANE, DEFA and ARG1 by specific antibodies
