Fig. 6: Relationships among GABA pathway metabolites and transcripts in ‘Empire’ apple fruit under low-temperature/low-O2 storage at two CO2 levels for up to 16 wk. | Horticulture Research

Fig. 6: Relationships among GABA pathway metabolites and transcripts in ‘Empire’ apple fruit under low-temperature/low-O2 storage at two CO2 levels for up to 16 wk.

From: Targeted quantitative profiling of metabolites and gene transcripts associated with 4-aminobutyrate (GABA) in apple fruit stored under multiple abiotic stresses

Fig. 6: Relationships among GABA pathway metabolites and transcripts in ‘Empire’ apple fruit under low-temperature/low-O2 storage at two CO2 levels for up to 16 wk.The alternative text for this image may have been generated using AI.

a Pearson’s correlation analysis. A total of 666 pairs were analysed, from which 68 resulted in significant correlations (P ≤ 0.05). Of these, 58 were positive and 10 negative. Each square represents the correlation between each metabolite or transcript in the column and the metabolite or transcript in the corresponding row with a color scale (color scale key at the side of the figure). b Principal component analysis. Score plot: fresh harvest control (*) and all variables at 2 (circles), 4 (triangles), 8 (squares) and 16 (inverted triangles) weeks of storage; 0.03 kPa CO2 (open symbols) and 5 kPa (filled symbols) CO2. The 2-week (early) and 8/16-week (late) harvests are enclosed separately by solid lines. c Principal component analysis. Loading plot of all variables: metabolites (open circles); transcripts (closed circles). The two principal components (PCs) explained 43.4% of the overall variance (30.2 and 13.2% for PC1 and PC2, respectively). The further from the origin, the more variables would be influenced by low temperature/low O2 (PC1) or elevated CO2 (PC2); variables with a higher loading value for PC1, but lower for PC2 would be more influenced by low temperature/low O2, whereas variables with a higher loading value for PC2 but a lower loading value for PC1 would be more influenced by elevated CO2. Ala alanine, ALA-T alanine transaminase, ALDH aldehyde dehydrogenase, AO, amine oxidase, GABA 4-aminobutyrate, GABA-T GABA transaminase, GAD glutamate decarboxylase, GHB 4-hydroxybutyrate, Glu glutamate, GLYR glyoxylate reductase, PAO polyamine oxidase, NAD+/NADH oxidized/reduced nicotinamide dinucleotide, NADP+/NADPH oxidized/reduced nicotinamide dinucleotide phosphate, NAD(H) total oxidized/reduced nicotinamide dinucleotide, NADP(H) total oxidized/reduced nicotinamide dinucleotide phosphate, NAD(P)(H) total oxidized/reduced nicotinamide dinucleotide (phosphate), PA total polyamines, Put putrescine, Spd spermidine, Spm spermine, SSADH succinic semialdehyde dehydrogense, Succ succinate; TAA total amino acids

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