Fig. 2

Intermediate state of NAT between healthy and tumor tissue. a t-SNE plots for each tissue types. Each group is clustered on its own in all plots. In 6 of 8 plots, the NAT samples (orange) are in between the healthy (green) and tumor (purple) samples. In bladder, there is not sufficient power of non-tumor samples compared with the tumor obstructing the discrimination between the conditions, yet the NAT samples are in between the tumor and the healthy tissue. Colon is an exception because of an issue related to the source of the healthy samples. b Deconvolution analysis of the NAT samples using median expression levels of healthy and tumor as references. The result of the analysis is the fraction of similarity of each NAT sample to the tumor. The small points, normal (green) and tumor (purple) deconvolution fractions, are shown as reference