Fig. 2

Schematic of the experimental setting and DoE raw data growth curves showing OD measurements. The DoE is based on a central composite design (figure in the upper left corner). NH₄Cl, CH₂O, and CH₃OH were used as factors during the experiment and systematically varied in a multivariate design space (see Supplementary Table 1 for the concrete values). Each of the factors setting was examined in triplicates. The centre point (O) was examined in quintuplicates. The colours of the dots and the letters of the figure in the upper left corner correspond to the growth curves. The line labelled ZC represents the optical density of a corresponding zero control experiment, which was done with the same medium as the experiments labelled with O (central point), but without inoculum. The different colours represent different performances. For better readability, the error bars in this diagram were excluded, which were in a standard deviation range between 0.0009 and 0.1544. According to statistical selection criteria three experiments (one experiment F and two experiments O) were excluded from ANOVA analysis (Supplementary Table 2)