Fig. 2
From: USP15-dependent lysosomal pathway controls p53-R175H turnover in ovarian cancer cells
MCB-613 causes lysosome-mediated degradation on p53-R175H. a Effect of MCB-613 (2 h) on p53 mRNA levels in ALST and TYK-Nu cells. Values are normalized mean ± s.e.m. (n = 3). b–d siRNA-mediated knockdown (48 h) of SRC-1 (b), SRC-2 (c), and SRC-3 (d) had no effect on p53-WT (ALST cells) and p53-R175H (TYK-Nu cells) levels. e shRNA-mediated knockdown of SRC3 (72 h) in TYK-Nu cells does not affect p53-R175H levels. f SRC-3 inhibitor SI2 (50 nM, 2 h) has no effect on p53-R175H levels in TYK-Nu cells. g, h MCB-613 caused a g decrease in the half-life of p53-R175H and h increase in the half-life of p53-WT. i Proteasome inhibitor MG132 does not inhibit MCB-613 induced turnover of p53-R175H. j Lysosome inhibitors (pepstatin A, Leupeptin, and E-64D) rescued MCB-613 induced turnover of p53-R175H
