Fig. 8

Adoptively transferred liver CD8 T cells from PMIF-immunized mice confer protection to homologous sporozoite challenge. a BALB/cJ mice immunized with replicons encoding Con RNA or PMIF RNA were infected with 2000 PbAWT sporozoites and cured by 6 days of chloroquine treatment (days 7–12). Four weeks later, the mice were reinfected with 2000 PbAWT sporozoites and T cells from liver isolated 7 days after infection, incubated with chloroquine to eliminate residual blood-stage parasites, and labeled with CFSE. Purified CD45.2⁺ CD8 T cells (2 × 10⁷) then were transferred into naïve congenic CD45.1 BALB/cJ hosts and the mice infected 3 days later with 2000 PbAWT sporozoites. b Luminescence values of infected mice and c parasitemia in mice adoptively transferred with liver CD8 T cells from Con RNA (white circle) or PMIF RNA (black circle) immunized mice. Results are from two separate experiments. Bars represent the mean of 6 mice ± SD **p < 0.01 by two-way ANOVA. d Representative CFSE dilution histogram of adoptively transferred (CD45.2) CD8 T cells from Con RNA or PMIF RNA immunized donors and enumeration of recovered CD45.2 CD8 T cells. e Number of proliferating CD45.2 CD8 T cells (CFSE^lo) producing IFN-γ after stimulation ex vivo with CSP peptide in the presence of Brefeldin A. Results are from two separate experiments. Bars represent the mean of 6 mice ± SD;**p < 0.01 by two-tailed Mann–Whitney test, error bars denote ±SD