Table 1 Representative RLU of DMSO and Epox-treated samples from different cell types

From: A novel probe to assess cytosolic entry of exogenous proteins

RLUa

Phagocytosis

Endocytosis

DMSO (x104)

Epox (x104)

DMSO (x104)

Epox (x104)

293T-FcRγIIA-Kb b

14.57

69.49

0.27

2.16

TRal c

0.24

0.33

0.06

1.01

293T d

0.07

0.14

0.04

0.19

Expi293F e

0.11

0.48

0.56

1.90

hDC f

0.23

2.24

0.11

1.32

BMDC g

0.75

8.82

22.59

24.03

BMDC h

0.22

9.06

18.69

18.29

  1. aRLU was measured directly from 10 μL total cell lysate. Mean value of background-subtracted normalized RLU from a representative experiment is shown
  2. b2 × 105 293T-FcRγIIA-Kb cells were assayed 6 h after feeding, and Epox was used at 200 nM for proteasome inhibition
  3. c2 × 105 TRal cells were assayed 6 h after feeding, and Epox was used at 800 nM
  4. d2 × 105 293T cells were assayed 6 h after feeding, and Epox was used at 200 nM
  5. e2 × 105 Expi293F cells were assayed 6 h after feeding, and Epox was used at 200 nM
  6. f2 × 105 hDCs were assayed 6 h after feeding, and Epox was used at 800 nM
  7. g5 × 105 BMDCs were assayed 3 h after feeding, and Epox was used at 800 nM
  8. h5 × 105 BMDCs were assayed 3 h after being fed ddRLuc-FcCHO−, and Epox was used at 800 nM
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