Fig. 3
Upon IFN priming, HSPC-pDCs can be modulated to become classical pDCs. a Graphical illustration of the experiment setup of HSPC-pDC priming. After enrichment, HSPC-pDCs were primed in medium supplemented with IFN-β + γ or IL-3 alone. After 72 h (day 21 + 3) primed cells were harvested for phenotypic or functional analyses. b Surface expression levels of pDC-related markers on HSPC-pDC cells after priming for 72 h with IFN-β + γ (Day 21 + 3IFN β+γ) and blood pDCs. c, d Type I IFN (c) or IL-6 (d) response of blood pDCs, unprimed or IFN-primed HSPC-pDCs cells to TLR7 and TLR9 agonists. e Type I IFN response of HSPC-pDCs primed with titrated concentrations of IFN-β + γ before being stimulated with the TLR7 agonist R837. Data shown are ± SEM of three donors. Statistical analysis was performed using regular two-way ANOVA (b–d) followed by Bonferroni post hoc test
