Table 2 Time course of mRNA expression in cultured myocardium

From: Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro

Function and protein

Gene

8 days

14 days

24 days

35 days

Intercept

Slope x35

Growth factors

  Growth differentiation factor 15

GDF15

2.98

2.07

2.26

1.32

3.19

−1.79

  Hepatocyte growth factor

HGF

2.21

3.12

2.66

3.17

2.30

0.84

  Opioid growth factor rec. like 1

OGFRL1

1.16

2.20

0.91

2.00

1.33

0.41

  Platelet derived growth factor receptor beta

PDGFRB

1.34

3.66

1.93

3.56

1.64

1.70

  Placental growth factor

PGF

3.06

4.20

2.42

3.65

3.38

−0.08

  Transforming growth factor beta 1

TGFB1

1.69

2.75

1.76

2.22

2.04

0.12

  Angiotensin I converting enzyme

ACE

1.87

3.02

1.94

2.05

2.43

−0.36

Extracellular matrix

  Collagen type I alpha 1 chain

COL1A1

3.16

4.04

2.90

3.86

3.31

0.32

  Collagen type I alpha 2 chain

COL1A2

2.42

3.19

2.20

3.36

2.40

0.68

  Collagen type III alpha 1 chain

COL3A1

2.25

3.30

2.50

3.52

2.26

1.09

  Vimentin

VIM

0.96

1.70

1.25

1.21

1.27

0.02

  Laminin subunit gamma 1

LAMC1

0.87

1.45

0.30

1.77

0.73

0.63

  Periostin

POSTN

2.70

3.48

1.92

3.44

2.73

0.26

  Fibronectin 1

FN1

3.24

3.89

2.41

3.35

3.49

−0.46

Hypoxia-induced targets

  Vascular endothelial growth factor A

VEGFA

−0.59

1.18

−0.98

−0.38

0.26

−0.78

  Solute carrier family 2 member 1

SLC2A1

−1.97

1.79

−1.30

−0.12

−0.70

0.53

  Glyceraldehyde-3-phosphate dehydrogenase

GAPDH

−0.20

−0.19

−0.24

−1.02

0.18

−1.02

  Protein kinase, cAMP-activated

PRKACA

−0.33

−0.78

−1.11

−0.62

−0.50

−0.37

  Phosphoglycerate kinase 1

PGK1

−0.85

0.18

−0.80

−0.94

−0.24

−0.63

Positive trend

  Elastin

ELN

−2.68

−1.10

−0.75

2.12

−3.88

5.67

  Nerve growth factor receptor

NGFR

−1.80

2.04

0.91

3.71

−2.03

5.61

  Kelch-like family member 38

KLHL38

−3.53

−0.90

−1.16

1.48

−4.22

5.52

  X-prolyl aminopeptidase 2

XPNPEP2

−1.77

2.22

1.05

3.65

−1.87

5.45

  Integrin subunit beta like 1

ITGBL1

−2.75

0.02

−0.20

2.16

−3.30

5.38

  Neurotrophic receptor tyrosine kinase 2

NTRK2

−1.88

−0.43

−0.61

2.45

−2.98

4.95

  Phospholipid phosphatase rel. 4

LPPR4

−0.39

0.67

2.76

2.60

−0.96

4.10

Negative trend

  Prolyl 4-hydroxylase alpha 3

P4HA3

3.61

4.06

2.55

0.40

5.26

−4.51

  Interleukin 6

IL6

2.56

1.20

1.13

−1.18

3.43

−4.33

  TNF receptor superfamily member 12 A

TNFRSF12A

2.71

−1.30

−0.96

−1.48

2.18

−4.21

  Cell division cycle associated 2

CDCA2

1.94

0.23

−0.48

−1.50

2.41

−4.08

  Wnt family member 5 A

WNT5A

2.90

1.19

0.20

−0.21

3.20

−3.77

  Protein phosphatase 1 regulatory subunit 16 B

PPP1R16B

0.21

−0.31

−2.56

−2.36

0.91

−3.75

  C-X-C motif chemokine ligand 1

CXCL1

3.15

1.10

0.25

0.02

3.26

−3.68

  1. Gene expression reflecting myocardial remodelling during tissue culture. The ratios of gene transcripts in cultured vs. uncultured tissue from the same patient are expressed as Log2-values. Temporal alterations of gene expression were approximated by linear regression of all culture durations. This calculation estimated the change of expression levels at the start of cultivation (intercept), and the temporal trend during 35 days of culture (slope ×35). Genes were selected that represent typical culture-related alterations, such as cell proliferation and hypoxia. Exemplary genes are also shown that underwent most extensive induction or downregulation in the course of biomimetic culture (positive or negative trends). Complete mRNA expression data are provided as Supplementary Data 1
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