Fig. 5 | Nature Communications

Fig. 5

From: Barcode-free next-generation sequencing error validation for ultra-rare variant detection

Fig. 5The alternative text for this image may have been generated using AI.

Identification of true variants reduction by quality control through the barcode-free NGS error validation. a Comparison of variants after filtering raw data with the Q-score (>Q10, >Q20, and >Q30). Variants before the NGS error validation were reduced (3.26 times less) more than variants after validation (2.48 times less), which might include low Q-score of variant calls. b Reduction of true variants by quality control from >Q10 to >Q30. From Q-score over than 18, the true variants were confirmed to decrease by the barcode-free NGS error validation. Source data are provided as a Source Data file

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