Fig. 2
From: Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
Cumulative editing and analysis of lineage tracing experiment. a Cumulative editing efficiency in the target regions using sgRNA-1 and sgRNA-3 for the defined time points. b A representation of the tree experiment for sgRNA-3 in HEK293T cells. The single-cells were sorted and expanded to a limited number of cell divisions. The bulk cells were then amplified from a 96-well plate and sequenced for cell lineage analysis. A network graph7 was constructed from all the pairwise cell barcode information. The “−” represents the mother-child node relationship and the colors of each circle represent the different clade (pink: Clade1, yellow: Clade2, blue: Clade3, green: Clade4). The misplaced nodes are represented by a red arrow. The red solid line connects the incorrectly placed mother-daughter node, and the dotted line indicates the correct mother-daughter node connection. c Time-lapse imaging of the single-cell expansion experiment. At the end of the video, single-cells were picked and sequenced for lineage reconstruction. d An example of a tree expansion experiment for seven single-cells. The bootstrapped confidence score (bootstrapped P-values × 100, n = 1000) are shown at the branching point. Physical isolation was impossible for the left most cell (1–1–1) in (c). The source data are available in the Source Data file
