Fig. 3
Extracellular electron uptake leads to a reducing intracellular redox environment. a TIE-1 WT NADH/NAD+ and b NAD(P)H/NAD(P)+ ratios under various growth conditions. Conditions tested: yeast-extract peptone (blue); photoheterotrophy with acetate (red) and butyrate (green); and photoautotrophy with H2 (yellow) or a poised electrode (black). Data are means ± s.e.m. of three biological replicates assayed in triplicate. The P values were determined by one-way ANOVA followed by a pairwise test with Bonferroni adjustment (*P < 0.05, **P < 0.01, ***P < 0.0001; ns, not significant). c Transcriptomic analysis of the de novo NAD biosynthesis pathway under various photoautotrophic and photoheterotrophic growth conditions. d Genome-wide transcriptomic analysis of NAD(P)+/H-requiring reactions. Source data (and reactions not mentioned in text) are provided as a Source Data File
