Fig. 4
From: Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway
Progression of H/D exchange in 3D R3-CANCO spectra. 2D planes from selected regions of the 3D R3-CANCO spectra illustrate the effect of R3 filter and the H/D exchange as a function of incubation temperature on spectral intensities for selected residues: K210 and G212 (TM region of helix G), A55 (extracellular end of helix B), P33 (A-B loop), P81 (TM region of helix C). Splitting of the peaks (red and blue) indicate multiple local conformations resulting from the heating/cooling cycle. These conformations are however similar based on the small chemical shift differences. The first contour is at 5 times root-mean-square of the noise. Buffer conditions (H2O or D2O), incubation temperatures, and R3 filter conditions are indicated in the top row. All spectra were collected on sample 1
