Fig. 4 | Nature Communications

Fig. 4

From: Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli

Fig. 4The alternative text for this image may have been generated using AI.

Translation-dependent gene loci movement toward the nucleoid periphery in E. coli RNAP transcription. a 5′ UTR sequence of the mutated strains. The underlined AGGAAA in the WT strain (lacZ-12xTetO) was mutated to TCTCTC (red letters) (ΔRBS, lacZ-12xTetO_ΔRBS) for RBS substitution. The start codon of the lacZ gene (underlined) was mutated to TAA (ΔRBSΔATG, lacZ-12xTetO_ΔRBS_ΔATG). b β-galactosidase assay showing that LacZ expression in the strain in which the start codon in the ΔRBS strain was replaced by a stop codon (ΔRBSΔATG) decreased to ~20% of that in the ΔRBS strain (blue bars, 1 mM IPTG induction of each strain). The LacZ expression level in ΔRBSΔATG cells was lower than that in WT cells under repressed conditions (no IPTG, gray bar). c Average x-positions of the lacZ gene locus were determined in the absence (repressed, gray bars) and presence of IPTG (induced, blue bars). d The degree of gene movement is the difference in the average position before (repressed) and after (induced) induction in c. Bar and error bars represent mean ± s.d. from three independent experiments. Each point represents independent measurement

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