Fig. 5: Knockdown of circGRIA1 increases Gria1 expression.

a BASEscope ISH showing circGRIA1 expression in macaque hippocampus s. Ten-micron cryostat sections were performed BASEscope ISH (red dots with nuclei counterstaining by hematoxylin). Scale bar, 50 μm. The images are representative of three independent experiments of each sample from 2 to 3 animals per age group. b Relative intensities of BASEscope ISH signals of circGRIA1 illustrated in (a) were quantified by use of Image J. Data are present as mean ± S.D. (n = 35–45 cells per group; *p < 0.05, unpaired t-test). c RT-qPCR was performed for validation of circGRIA1 expression. Data are presented as mean ± S.D.; n = three independent experiment of each sample from 2 to 3 animals per age group; *p < 0.05, unpaired t-test. d RNAscope ISH showing Gria1 mRNA expression. Ten-micron cryostat sections were performed RNAscope ISH (green dots with nuclei counterstaining by DAPI). Scale bar, 100 μm. The images are representative of three independent experiment of each sample from 2 to 3 animals per age group. e Relative intensities of RNAscope ISH signals of Gria1 illustrated in (d) were quantified by use of Image J. Data are present as mean ± S.D. (n = 60–75 cells per group; *p < 0.05, unpaired t-test). f Total RNAs were extracted from postmortem frozen hippocampal tissues of 10- and 20-year-old female macaques with microinjection of viral particles containing siRNAs against circGRIA1 or siRNA-control into hippocampus of macaque brains as indicated. qPCR was performed for validation of Gria1 expression. Data are presented as mean ± S.D.; n = three independent experiments of each sample from 2 to 3 animals per age group; *p < 0.05, unpaired t-test, error bars denote S.D. Source data are provided as a Source Data file.