Fig. 6: DegP2 influences the intersection of heme biosynthesis, the TCA cycle, and the ETC.

a TPP revealed that depleting DegP2 reliably changed the melting temperatures of 13 proteins (highlighted in green, p < 0.2 by z-test in each of two replicates). Three mitochondrial proteins are indicated by their gene IDs. b Melting curves for three mitochondrial proteins identified as hits by TPP. c Dose–response curve for parasites treated with the complex II inhibitor TTFA. Results are mean ± SEM for n = 4 or 3 independent experiments, for the parental and ∆DegP2 or ∆DegP2/DegP2-HA strains, respectively. ∆DegP2 is significantly more resistant to TTFA compared to the parental; p < 0.0001 from extra-sum-of-squares F-test. d Dose–response curve for parasites treated for 5 h with increasing concentrations of atovaquone (ATQ). Results are mean ± SEM for n = 5 or 3 independent experiments for the parental or ∆DegP2 strains, respectively. Both strains displayed similar susceptibility to ATQ; p = 0.59 from extra-sum-of-squares F-test. e Summary of changes in metabolites between parental and ∆DegP2 parasites in the TCA cycle and closely related pathways. Full results can be found in Supplementary Data 6. Asp aspartate, α-KG α-ketoglutarate, Gln glutamine, Glu, glutamate. Asterisks indicate significant change from parental, *p < 0.05, **p < 0.005, ***p < 0.0005, by two-way ANOVA.