Fig. 1: Distribution of SNARE proteins at the docking interface differs between loose and tight docking states.
From: Tight docking of membranes before fusion represents a metastable state with unique properties
a Schematic illustration showing loose and tight docking states (left) and the experimental system used in the docking experiments (right). One set of GUVs contain the lipidic membrane dye DiD (magenta) and unlabeled Q-SNARE complexes, whereas the other set of GUVs contains the synaptobrevin docking mutant ∆84 syb, labeled with Oregon Green 488 (OG, green). SNARE complex structure PDB ID: 3IPD39. b, c Examples (from >5 independent experiments) of a loosely (b) and tightly (c) docked GUV pair, respectively, imaged by fluorescence microscopy. The position of a line scan for fluorescence intensities (graph next to images) is marked with a yellow line. Labeled synaptobrevin is enriched at the interface in the loosely docked state and depleted at the interface in the tightly docked state (see also schematic drawing on the right). Scale bars 5 μm. Source data are provided as a Source Data file.
