Fig. 3: Membrane thickening at tight docking interfaces.
From: Tight docking of membranes before fusion represents a metastable state with unique properties
a Membrane thickness of loosely (top) and tightly (bottom) docked interfaces, measured by line scans (at the interfaces) and radial profile analysis (membranes outside docking interfaces). Thickness is determined as distance between peaks corresponding to lipid headgroups between the two leaflets. Scale bars 50 nm. b Comparison of membrane thicknesses at tight (n = 50), loose (n = 72), and docking-free interfaces (n = 122) obtained by analyzing cryoEM images as shown in a. Boxes represent interquartile range, and whiskers below and above indicate full data range. Line in a box represents median. Data were analyzed with a one-tailed unpaired t-test at α = 0.05, ****P < 0.0001; **P = 0.0015. c Membrane thickness variations within single membranes being involved in docking interfaces (int) or free (nTight = 50; nLoose = 72). Data were analyzed with the Wilcoxon matched-pairs signed-ranks test at α = 0.05, ****P < 0.0001; **P = 0.006. Source data are provided as a Source Data file.
