Fig. 4: Protein O-glycomics revealed a higher proportion of O-GalNAc glycans compared to O-Man in mouse brain.
From: Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues
A Representative MALDI spectra of protein O-glycans isolated from four regions show a consistent pattern across the brain. B The 10 most abundant O-glycan masses in the brain include both O-GalNAc and O-mannose (O-Man) type structures (CTX = 3, HIP = 2, STR = 4, CBLM = 4, male mice). Data presented as mean percent abundance +/− SEM. Corresponding glycan structures are presented above each peak, with distinct isomers corresponding to the same mass shown in brackets. For single-factor ANOVA calculations, df = (3,9), Fcrit = 3.862. p values *<0.05, **<0.01, and ***<0.001. C Categorical analysis of O-glycans revealed differences in the abundance of O-GalNAc, O-Man, and NeuGc-containing glycans between regions. Analyzed independently, O-GalNAc and O-Man glycans varied in their proportion of core 1, core 2, and sialylated structures. Data presented as a heat map showing percent change from the average of four brain regions. Source data are provided as a Source Data file.
