Fig. 7: Differential RNA expression between brain regions in mice spans multiple glycosylation pathways and correlates with glycomics results.
From: Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues
RNA seq data from cortex and cerebellum (n = 4 each, male mice) revealed differential expression of enzymes involved in several glycosylation pathways, including the synthesis of N-glycans (A), O-GalNAc glycans (B), and O-Man glycans (C). Transcripts in red have significantly increased expression in the cortex relative to the cerebellum, and those in the blue are decreased, as determined using the EdgeR method with gene cutoffs of 2-fold change in expression value and false discovery rates (FDR) below 0.05. *Tmtc1-4 add O-linked Man but these residues are not extended further. Mouse brain RNA seq results for N-acetylglucosaminyltransferases (D), fucosyltransferases (E), and O-Man specific enzymes (F) demonstrated high RNA levels of Mgat3, Fut8, Fut9, and Mgat5b, which correlate with results from glycomics. Data for TPM presented as mean +/− SEM. For D-F, n = 4 independent samples for each brain region from different mice. Human RNA seq data from GTEx portal showed a similar expression profile for N-acetylglucosaminyltransferases (G), fucosyltransferases (H), and O-Man pathway enzymes (I) in the brain between humans and mice, but this pattern is distinct from human liver and lung. Source data are provided as a Source Data file.
