Fig. 1: CinA mediates intrinsic isoniazid tolerance.

a Bacteria were starved in PBS for 14 days and then exposed to isoniazid (INH, 0.5 µg/ml), rifampicin (RIF, 1 µg/ml), or an equal amount of DMSO for 7 days and cultured for CFU enumeration. Data are means ± SD of triplicate cultures and are representative of two independent experiments. b CFU from resting and c IFN-γ activated primary murine BMDMs infected with the indicated strains and treated with INH (0.1 µg/ml), RIF (0.1 µg/ml), or an equal amount of DMSO from 24 to 120 h post infection. Data are means ± SD of triplicate cultures and are representative of two independent experiments for c. d Impact of isoniazid and rifampicin on growth of the indicated strains. Data are means ± SEM from two independent experiments, each performed with duplicate cultures. e CFU quantification of the indicated strains after incubation with 0.5 µg/ml isoniazid in standard growth media. Data are means ± SEM from two independent experiments each performed with triplicate cultures. Statistical significance of the differences between wild-type and ΔcinA was assessed by two-tailed, unpaired t-test, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.