Fig. 4: Spatial analysis of the immune cell repertoire.

a Spatial mapping of the signature genes related to B cell maturation (n = 106 biologically independent samples (ROI)). Bar plot indicates mean value and error bar indicates standard deviation. b Six representative BCR sequences recovered using Select-seq and matched for gene expression related to B cell maturation. Overall, both heavy and light chain sequences from 42.9% of samples were recovered using Select-seq. Representative sequences including isotype, CDR3, matched V and J genes for each heavy and light chain. c Matched spatial context of recovered BCR sequences. Most of the BCRs were detected in the CD44low/−/ALDH1high stromal region, and very few BCRs were detected in the CD44high/ALDH1low/− duct region. Four of the six HCDR3 amino acid sequences were recovered from the CD44−/low/ALDH1high region, and two were recovered from the CD44−/ALDH1− region. d Single-cell deconvolution of each ROI (left) and ROI group (right). Source data for b–d are provided as a Source Data file.