Fig. 1: Nucleoli are fused in later stages of infection.
From: The nucleolus is the site for inflammatory RNA decay during infection
a Still images from time-lapse sequences showing nucleolar fusion in FBL-GFP-expressing RAW 264.7 macrophages by analyzing patterns of FBL through LPS (80 ng ml−1) stimulation. Dashed lines delineate the borders of same cell for chasing nucleolar fusion in a cell. b Graphs showing the percentage of cells with one or more than two nucleoli (left) or the volume of nucleoli (right). Dot plot is presented as means ± s.e.m. c Images showing the nucleolar morphology in RAW 264.7 cells after 12 h LPS stimulation or recovery from LPS treatment. The dashed line delineates the borders of cells. Graphs showing the percentage of cells with one or more than two nucleoli. For details on experimental design of recovery from LPS stimulation, see Supplementary Fig. 1c or Methods. d–f Images showing nucleolar fusion at the indicated times of LPS stimulation in BMDMs or BMDCs (d), and in RAW 264.7 cells stimulated by CpG-DNA (1 μM) or Poly(I:C) (100 μg ml−1) for 18 h (e), or infected with E. coli for 15 h (f). Graphs representing the percentage of cells with indicated nucleoli numbers per cell. n, total cells counted. Green FBL, blue DAPI. Scale bars, 5 μm. P values are determined by unpaired two-tailed t test. **P < 0.01. All data are representative of three independent experiments and are presented as means ± s.d. in (b–f). Source data are provided as a Source Data file.
