Fig. 2: Genetic evidence and effects of OsGF14h in response to the anaerobic stress.

a qRT-PCR-based transcript abundance analysis of OsGF14h in organs and tissues of WR04-6, including the germinating embryo and endosperm, developing seeds, radicle, stem, leaf, spikelet, and inflorescence meristem. DAI days after imbibition, DAF days after fertilization. Data were presented as mean ± SD, n = 3 biologically independent samples. b Subcellular localization of OsGF14h^WR04-6-GFP fusion protein in rice protoplasts. Scale bars = 10 μm. A representative experiment from three independent experiments is shown. c Performance of anaerobic germination between WT^SN9816 and its overexpression lines (OxOsGF14h^SN9816-5 and OxOsGF14h^SN9816-11), between WT^SN9816 and its overexpression lines (OxOsGF14h^WR04-6-8 and OxOsGF14h^WR04-6-25), and between WT^WR04-6 and its CRISPR/Cas9 knockout lines (gf14h-13 and gf14h-15) after 4 days of anaerobic treatment. Scale bar, 1 cm. d The comparisons of coleoptile lengths between WT^SN9816 and its overexpression lines OxOsGF14h^SN9816, between WT^SN9816 and OxOsGF14h^WR04-6, and between WT^WR04-6 and its knockout lines after 4 days of anaerobic germination, respectively. Data were presented as means ± SD. n = 5 independent experiments. P values are indicated by an unpaired two-tailed Student’s t-test. e Performance of anaerobic seedling development between WT^SN9816 and overexpression lines OxOsGF14h^WR04-6, between WT^WR04-6 and knockout lines under the flooded direct-seeded condition on the 21st day. Scale bar, 1 cm. f Performance of flooding adaptation of WT^SN9816 and its overexpression line OxOsGF14h^WR04-6 in flooded tanks under flooded direct-seeded condition (a water layer of 10 cm was maintained); the photo was taken on the 21st day after flooding. g The seeding rate of WT^SN9816 and OxOsGF14h^WR04-6 seedlings. Data were presented as means ± SD. n = 3 independent measurements in three flooded tanks. P values are indicated by an unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.