Fig. 4: OsGF14h^WR04-6 inhibits ABA response and enhances GA biosynthesis.

a The relative expression of ABA regulated and responsive genes between WT^WR04-6 and OsGF14h knockout lines, between WT^SN9816 and OsGF14h overexpression lines under anaerobic (blue background) and aerobic conditions at the 12 h. b Assay of exogenous ABA with gradient concentrations to reflect the ABA sensitivity for OsGF14h knockout lines and WT^WR04-6. Error bars indicate the means ± SD of three independent experiments. c Comparison of relative germination rate between WT^SN9816 and two types of overexpression lines (function and partial loss-of-function) and between WT^WR04-6 and OsGF14h knockout lines under 72 h of anaerobic incubation with exogenous 1 µM ABA treatment. In b, c seeds with the embryo showing white without the emergence of coleoptile are still considered as germination. d The expression model of six DEGs on the GA biosynthesis pathway is presented based on RNA sequencing. The left three and right three rectangles under the gene names (highlighted in red) represent the FPKM value of the three biological replicates of OxOsGF14h^WR04-6 and OxOsGF14h^SN9816, respectively, in response to anaerobic stress. The color scale indicates low (blue) to high (red) FPKM value. e The OsGA20ox1 expression level between WT^WR04-6 and OsGF14h knockout lines and between WT^SN9816 and functional OsGF14h overexpression lines at the 12th hour under anaerobic (blue background) and aerobic conditions. f Heatmap of three kinds of endogenous GA content in WT^WR04-6 and OsGF14h knockout line gf14h-15 during anaerobic germination. The color key (blue to red) represents the GAs content from low to high value. g Performance and coleoptile lengths of WT^WR04-6 and OsGA20ox1 knockout lines on the 4th day of anaerobic germination. Scale bar = 1 cm. In a, c, e, g, data were presented as means ± SD, P values are indicated by an unpaired two-tailed Student’s t-test. In a, e, f n = 3 biologically independent samples. In b, c, n = 3 independent experiments. In g n = 5 independent experiments. Source data are provided as a Source Data file.