Fig. 1: ADAM10 is upregulated in experimental heart injury as well as patients with ischemic cardiomyopathy and correlates with ANP/ BNP expression.

a Western blot analysis and b quantification of indicated ADAMs in heart tissue lysates of sham-operated (Sham) and LAD-ligated (MI) mice 3 days after infarction (n = 5, mean ± SEM, ns not significant, *P < 0.05, two-tailed Mann–Whitney test). Exact P-values: ADAM8, 0.150794. ADAM10, 0.015873. ADAM12, 0.150794. ADAM17, 0.690476. ADAM19, 0.690476. c Western blot analysis of ADAM10 in heart tissue lysates from patients with ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM) versus non-failing controls (NF) (n = 6). Pearson’s correlation of ADAM10 mRNA expression against expression of d NPPA (ANP) and e NPPB (BNP) (n = 12). Exact P-values: NPPA, <0.0001. NPPB, 0.0008. f Western blot analysis and g quantification of ADAM10 in heart tissue lysates of mice 14 days after sham-operation (Sham) and LAD-ligation (MI) (n = 6, mean ± SEM, *P = 0.011, two-tailed t test). h Immunofluorescence images and quantification of ADAM10 (A10), cardiac Troponin T (cTnT, cardiomyocytes, CM), Vimentin (Vim, fibroblasts, FB), CD31 (endothelial cells, EC) and CD45 (immune cells, IC) stained heart tissue sections of sham-operated (Sham) and LAD-ligated (MI) mice 14 days after surgery. Nuclei are stained with DAPI. Representative images are shown. Scale bar – upper panel, 20 µm. Scale bar – lower panel, 10 µm. Dotted line indicates the infarct area. n = 4 per group, mean ± SEM, ns not significant, *P < 0.05, two-tailed Mann−Whitney test. Exact P-values: CM, 0.028571. FB, 0.190476. EC, 0.714286. IC, 0.904762. i Gene expression signature of ADAM10 (A10) in indicated cell types of human hearts. Publicly available single cell sequencing data of the “Heart Cell Atlas” were analyzed. Source data are provided as a Source Data file.