Fig. 6: Cardiomyocyte-specific ADAM10 KO improves post-MI cardiac function.
a–c Echocardiographic assessment of fractional area shortening, ejection fraction and left ventricular end-systolic interior diameter in 8-week-old ADAM10fl/fl (WT) and (αMHC-Cre) ADAM10 KO mice under a basal conditions (WT, n = 7, KO, n = 8, mean ± SEM, ns not significant) as well as b 3 days (WT, n = 8, KO, n = 9, mean ± SEM, ns not significant, *P < 0.05, two-tailed t test) and c 14 days after myocardial infarction (WT, n = 6, KO, n = 9, mean ± SEM, ns not significant, *P < 0.05, two-tailed t test, exact P-values are provided in the Source Data file). d Representative end-systolic B-mode and M-mode echocardiograms of ADAM10 WT and ADAM10 KO mice 14 days after myocardial infarction. e Western blot analysis and f quantification of ADAM10, CX3CL1 and IL-1β expression in heart tissue lysates of ADAM10 WT and ADAM10 KO mice 3 days after myocardial infarction (n = 6, mean ± SEM, ns not significant, *P < 0.05, **P < 0.01, two-tailed t test). Exact P-values: ADAM10, 0.0159. CX3CL1, 0.0084. IL-1β, 0.0317. g Analysis of leukocyte counts in blood samples of ADAM10 WT (n = 8) and ADAM10 KO (n = 7) mice (mean ± SEM, ns not significant, *P < 0.05, two-tailed t test). Exact P-values: Neutrophils, 0.0404. Eosinophils, 0.7886. Monocytes, 0.3373. Lymphocytes, 0.751. h Analysis of leukocyte counts in the infarcted area/ infarct border zone of ADAM10 WT (n = 8) and ADAM10 KO (n = 7) mice (mean ± SEM, ns not significant, *P < 0.05, two-tailed two-tailed t test). Exact P-values: Neutrophils, 0.0214. Eosinophils, 0.176. Monocytes, 0.639. Macrophages, 0.6064. Source data are provided as a Source Data file.
