Fig. 1: Changes to pre-40S ribosomal complexes during lytic KSHV infection determined by quantitative mass spectrometry. | Nature Communications

Fig. 1: Changes to pre-40S ribosomal complexes during lytic KSHV infection determined by quantitative mass spectrometry.

From: Kaposi’s sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs

Fig. 1: Changes to pre-40S ribosomal complexes during lytic KSHV infection determined by quantitative mass spectrometry.The alternative text for this image may have been generated using AI.

a Temporal Association of the 40 S ribosome biogenesis factor bait proteins during the maturation of the 18 S rRNA. b Whole cell lysates from latent and 24 h post lytic reactivation control TREx BCBL1-Rta cell line or TREx BCBL1-Rta cell lines stably expressing pre-ribosome biogenesis bait proteins were subject to Twin-Strep-tag® pulldowns. Silver stain gels of isolated pre-40S ribosome complexes. Representative stained gel is shown (n = 2 biologically independent samples). c–f TMT LC-MS/MS was used to identify the association and stoichiometry of all proteins present in the isolated complexes. Incorporation of each protein into the pre-40S ribosomal complexes isolated from cells infected with latent KSHV (x-axis) compared to lytic KSHV (y-axis) A minimum cut-off for incorporation was set to 100 abundance indicated by the grey boxes. A minimum threshold for increase and decrease incorporation of proteins into complexes isolated from lytic KSHV cells was set using the equations Y = X + 0.25 and Y = X−0.25 respectively. g Ratio increases in incorporation of two select groups of ribosome biogenesis in pre-40S ribosomal complexes isolated from cells infected with lytic KSHV compared to latent KSHV ∞ = only detected in lytic pre-40S complexes. * = below 100 abundance.

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