Fig. 3: Mutational analyses of Pla1-Red1 interaction. | Nature Communications

Fig. 3: Mutational analyses of Pla1-Red1 interaction.

From: Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex

Fig. 3: Mutational analyses of Pla1-Red1 interaction.The alternative text for this image may have been generated using AI.

Isothermal titration calorimetry experiments with specific Pla1 and Red1 point mutants that affect their interaction interface are shown in panels A–F. The calculated dissociation constant (KD) from an average of two independent measurements is shown. G In vitro polyadenylation assay. Polyadenylation of a 5′-Cy3 labelled A15 RNA primer by Pla1 alone (left panel), in the presence of Red1288-345 (middle panel) and Red1W298A/F305A (right panel), analysed by 14% denaturing urea PAGE at different time points. Densitometric analyses of the gels are plotted below, where the RNA length is marked based on an RNA ladder. Representative gels from two independent experiments are shown. Source data are provided as a Source Data file.

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