Fig. 4: Blocking MAIT cell activation impacts on the Ly6Chi vs Ly6Clo macrophage signature.
From: MAIT cell inhibition promotes liver fibrosis regression via macrophage phenotype reprogramming
a Heatmap of the 2687 genes with a significant (p ≤ 0.05, paired t test) difference in the ratio of Ly6Chi Ac-6-FP/vehicle and Ly6Clo Ac-6-FP/vehicle and a fold change ≥ 1.5. Clustering used Euclidean distance and Ward.D2 agglomeration method. b Top KEGG pathways from the enrichment analysis based on the 2687 genes with a significant difference in the ratio of Ly6Chi Ac-6-FP/vehicle vs Ly6Clo Ac-6-FP/vehicle. KEGG pathways are ordered by −log10(FDR). c Ac-6-FP/mean vehicle ratios in Ly6Chi (red) and Ly6Clo (blue) for selected genes from Apoptosis (mmu04210) and d Autophagy (mmu04140) KEGG pathways. e Geometric Mean of LC3II-positive cells in total macrophages (F4/80 + CD11b + ; *p = 0.03) and Ly6Chi and Ly6Clo (*p = 0.05) macrophages sorted from C57BL/6 J mice either injected Ac-6-FP or its vehicle. Statistical analyses were performed using two-tailed Mann–Whitney test. f Ac-6-FP/mean vehicle ratios in Ly6Chi (red) and Ly6Clo (blue) for selected genes from Glycerophospholipid metabolism (mmu00564). c–f Experiments were performed on n = 5 mice/group. Data are presented as mean values ± S.E.M. Up upregulated genes, Down downregulated genes. Source data are provided as a Source Data file.
