Fig. 5: KIAA1199 KO increases osteogenic potential of bone marrow stromal stem cells, enhances the osteogenic differentiation but inhibits the osteoclast differentiation. | Nature Communications

Fig. 5: KIAA1199 KO increases osteogenic potential of bone marrow stromal stem cells, enhances the osteogenic differentiation but inhibits the osteoclast differentiation.

From: KIAA1199 deficiency enhances skeletal stem cell differentiation to osteoblasts and promotes bone regeneration

Fig. 5: KIAA1199 KO increases osteogenic potential of bone marrow stromal stem cells, enhances the osteogenic differentiation but inhibits the osteoclast differentiation.The alternative text for this image may have been generated using AI.

A The changes of cell number of bone marrow stromal stem cells (mBMSC) from WT and KIAA1199 KO female mice were determined on day (D) 0 and 9, n = 4. B, C Colony-forming unit fibroblasts (CFU-Fs) were counted (B), CFU-ALP + colonies on day 7 were presented as a percent of CFU-Fs, n = 6. D, E Alkaline phosphatase (ALP) activity and ALP staining on day 10 and alizarin red (AZR) staining on day 18 during osteoblast (OB) differentiation of mBMSC (D); Real-time PCR analysis of OB gene markers: Alp, alkaline phosphatase, Bglap osteocalcin, Col1a, collagen type 1, Opn, osteopontin, Runx2, Runt-related transcription factor 2, Opg, osteoprotegerin, Rankl, Receptor activator of nuclear factor kappa-Β ligand (E), n ≥ 3. F, G Dynamic bone histomorphometry were measured in proximal tibia. Mineralized surface per bone surface (MS/BS, %), mineral apposition rate (MAR, µm/d), bone-formation rate per bone surface (BFR/BS, µm3/µm2/year) were compared between KO and WT male mice, n values are as labeled. H, I Osteoclast differentiation of bone marrow cells from female KIAA1199 KO and WT mice. The formation of multinucleated OC was verified by positive staining for tartrate-resistant acid phosphatase (TRACP) on day 5 (H). The expressions of OC maker genes were analyzed by real time PCR: Rank, tumor necrosis factor receptor superfamily member 11 A, Trap, tartrate-resistant acid phosphatase type 5, Ctsk, cathepsin K, Crl, calcitonin receptor (I), n ≥ 3. J Number of osteoclasts per bone surface (NOc/BS, /mm) by bone histomorphometry on mice tibias, n values are as labeled. K–M Plasma levels of procollagen type 1 N-terminal pro-peptide (P1NP) (K), C-telopeptide of type I collagen (CTX1) (L) and tartrate-resistant acid phosphatase 5b (TRAcP 5b) (M) in KIAA1199 KO and WT mice were measured by ELISA, n values are as labeled. Scale bar (F, H) = 100 µm, date is presented as mean ± SD, statistical difference between WT and KO was determined by two-tailed unpaired Student’s t-test. *P < 0.05 and **P < 0.01. Source data are provided as a Source Data file.

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