Fig. 8: Endogenous clients of TMX3, TMX4 and TMX5. | Nature Communications

Fig. 8: Endogenous clients of TMX3, TMX4 and TMX5.

From: TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress

Fig. 8: Endogenous clients of TMX3, TMX4 and TMX5.The alternative text for this image may have been generated using AI.

a Anti V5-antibody was used to immunoisolate from HEK293 cell lysates V5-tagged versions of TMX3 (lane 2), TMX3C56A (lane 3), TMX4 (lanes 4), TMX4C67A (lanes 5) and TMX5 (lanes 6). Part of the immunoisolates has been separate in non-reducing/reducing gel (lanes 1–6 and 7–12, respectively). The gel has been silver stained. Uncropped gel in Supplementary Fig. 6. The polypeptide bands in the black and red rectangles are disulfide-bonded complexes (mixed disulfides) associated with the respective TMX protein. The complexes disappear (yellow boxes), i.e., are disassembled, when the samples are run under reducing conditions (lanes 7–12). Part of the immunoisolates has been processed for mass spectrometry (see “Methods” section) to determine the composition of the mixed disulfides and identify the endogenous proteins trapped in mixed disulfides with the given TMX protein. b Graphical representation of Supplementary Table 1. Red dots show NESPRIN proteins.

Back to article page