Fig. 3: The FBF-2 CT autoinhibits RNA-binding affinity. | Nature Communications

Fig. 3: The FBF-2 CT autoinhibits RNA-binding affinity.

From: Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding

Fig. 3: The FBF-2 CT autoinhibits RNA-binding affinity.The alternative text for this image may have been generated using AI.

Representative EMSA gels and binding curves are shown for binding to FBEa RNA (5′-AUCAUGUGCCAUAC-3′) by a FBF-2 RBD, b FBF-2 RBD + CT, c FBF-2 RBD + CT Y479A, and d FBF-2 RBD + CT L610A. The left lanes are RNA only. Data points on graphs are shown as filled circles. We observed an intermediate band (*) that appears to be a non-specific interaction of FBF-2 RBD + CT that is not observed for the RBD or mutant proteins. We included this band as part of the unbound RNA. Mean Kd from at least three independent technical replicates with similar results are indicated (Number of replicates: FBF-2 RBD, n = 4; FBF-2 RBD + CT, n = 4; FBF-2 RBD + CT Y479A, n = 3; and FBF-2 RBD + CT L610A, n = 5). See also Supplementary Table 4. Source data are provided as a Source Data file.

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