Fig. 6: LST-1 A and B release autoinhibition and increase RNA-binding affinity of FBF-2 RBD + CT. | Nature Communications

Fig. 6: LST-1 A and B release autoinhibition and increase RNA-binding affinity of FBF-2 RBD + CT.

From: Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding

Fig. 6: LST-1 A and B release autoinhibition and increase RNA-binding affinity of FBF-2 RBD + CT.The alternative text for this image may have been generated using AI.

Representative EMSA gels and binding curves are shown for binding to FBEa RNA by a FBF-2 RBD + CT in the presence of 150 μM LST-1 A and b FBF-2 RBD + CT in the presence of 50 μM LST-1 B. The concentrations of LST-1 A and LST-1 B were chosen based on the affinities for FBF-2; LST-1 A binds with weaker affinity than LST-1 B. The left lanes are RNA only. Data points on the graphs are shown as filled circles. We observed an intermediate band (*) that appears to be non-specific interaction of FBF-2 RBD + CT a or LST-1 B b, as it occurs at low protein concentration. We included this band as part of the unbound RNA. Mean Kd from three independent technical replicates with similar results are indicated. See also Supplementary Table 4. Source data are provided as a Source Data file. c Cartoon illustrating relief of FBF-2 CT autoinhibition by LST-1 A or LST-1 B.

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