Fig. 1: Study design and long-read data overview. | Nature Communications

Fig. 1: Study design and long-read data overview.

From: Detection of isoforms and genomic alterations by high-throughput full-length single-cell RNA sequencing in ovarian cancer

Fig. 1: Study design and long-read data overview.The alternative text for this image may have been generated using AI.

a Schematic of freshly processed HGSOC omentum metastases and patient-matched tumor-free distal omentum tissue biopsies, scRNA-seq. b Definition of SQANTI-defined isoform structural categories. c Proportions of isoform structural categories detected in merged metastasis and distal omentum samples. Percentage and total number of isoforms per category are indicated. d Proportions of unique reads attributed to isoforms detected in (c). Percentage and total number of UMIs per category are indicated. e Percentage of isoforms for which transcription start site is supported by CAGE (FANTOM5) data and transcription termination site is supported by polyadenylation (PolyASite) data, per isoform structural categories. “GENCODE.all” indicates all protein-coding isoforms in the GENCODE database, “GENCODE.FL” is a subset of ‘GENCODE.all’ containing only isoforms tagged as full-length, and “GENCODE.MANE” is a hand-curated subset of canonical transcripts, one per human protein-coding locus. f GENCODE-defined biotype composition of novel isoforms. g Biotype composition of the GENCODE database.

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