Fig. 3: Mechano-controlled reversible biocatalysis.
From: Shape morphing of hydrogels by harnessing enzyme enabled mechanoresponse
a Schematic illustration of fluorescence generation based on the activation of thrombin through mechanical stretching (top). Picture of the hydrogel before and after stretching (bottom). The fluorescence mainly appears in the stretching region. b Emission spectra of the fluorogenic substrate inside hydrogels before and after mechanical stretching. c Fluorescence intensities measured in hydrogels for various stretching strains ranging from 20% to 300%. d Measurements of the fluorescence of alternatingly stretched (150% strain) and unstretched hydrogels (the time intervals for all tests are 3 min). Each experiment was repeated three times independently with similar results. Data are presented as mean values with error bars representing the standard deviation of three independent replicates (n = 3 in each group). Source data are provided as a Source Data file. Thr-S thrombin-SNAP, Hir-S Hirudin-SNAP, Thr-V thrombin-SNAP-Vinyl, Hir-V hirudin-SNAP-Vinyl.
