Fig. 4: fCRISPR reveals heterogeneity of chromosomal dynamics.
a fCRISPR reveals heterogeneity of chromosomal dynamics of Chromosome 3. To uncover the dynamics information of chromosome loci, we transfected fCRISPR with tdTomato-tDeg reporter targeting to Chromosome 3 in U2OS cells. We observed three fluorescent puncta (white arrowheads) and their movements in the nucleus (white dotted line). The results show that three Chromosome 3 loci have different movement speeds, revealing the heterogeneity of the specific genome loci. the time interval is 0.3 s, the gradient colors of each trajectory represent the from 0 s to 9.9 s. Scale bar for fluorescence figure, 5 μm. Scale bar for the trajectories, 0.2 μm. See Supplementary Movie 1. Source data are provided as a Source Data file. b Averaged MSD curves of fCRISPR-labeled Chromosome 3 in U2OS cells. The colored shaded area represents the 95% fitting confidence interval. The microscopic diffusion coefficient (Dmicro) was 1.8 × 10−3 μm2/s, which represents the confined movement of Chromosome 3.   n= 30 cells were analyzed. Source data are provided as a Source Data file.
