Fig. 5: fCRISPR detects the telomere length in UMUC3 and RPE cell lines. | Nature Communications

Fig. 5: fCRISPR detects the telomere length in UMUC3 and RPE cell lines.

From: Fluorogenic CRISPR for genomic DNA imaging

Fig. 5: fCRISPR detects the telomere length in UMUC3 and RPE cell lines.The alternative text for this image may have been generated using AI.

a UMUC3 and RPE cells expressing fCRISPR with tdTomato-tDeg or conventional CRISPR with dCas9-GFP were imaged to determine telomere length. To image telomere, we transfected fCRISPR (top) and conventional CRISPR (bottom) in living UMUC3 (left) and RPE (right) cells. Scale bar, 5 μm. b Quantification of normalized telomere fluorescence labeled by fCRISPR or conventional CRISPR in UMUC3 and RPE cells. The mean fluorescence of telomere puncta labeled by fCRISPR in RPE cells showed ~3.11-fold higher than in UMUC3 cells. The mean fluorescence of telomere puncta labeled by conventional CRISPR in RPE cells showed ~2.85-fold higher than in UMUC3 cells. The shown dots represent the mean fluorescence of single cells. n = 400 cells per condition. Source data are provided as a Source Data file.

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