Fig. 3: Specific hormonal regulation in related cell types.
From: An organism-wide atlas of hormonal signaling based on the mouse lemur single-cell transcriptome
a, b, e–h Dot plots showing expression of hormone ligand, synthase, modulator, and receptor genes that are differentially expressed in a male germ cells across different stages of spermatogenesis, b epithelial cells along the spatial axis of the kidney nephron tubule, e endothelial cells along the arteriole-to-venule spatial axis of the kidney vasa recta, f CPN2hi vs CPN2lo subtypes of hepatocytes, g between metastatic tumor cells in the lung (L2) and the putative cell type of metastatic origin (MUC16+ non-ciliated uterus epithelial cells, L3), and h endothelial cells of the lung and brain compared to other organs. c Diagram of the nephron and vasa recta showing the cell types’ spatial distribution, related to (b, d, e). d UMAP visualization of vasa recta cells based on expression of all genes. The black line shows the detected arteriole-to-venule trajectory with the arrow pointing in the direction of blood flow. Cells are color-coded according to the cell-type annotation names in the atlas. Gray lines show the alignment of the individual cells to the trajectory. In a, cell types are arranged along the course of the spermatogenesis. b Cell types are arranged according to their spatial distribution along the kidney nephron tubule. Solid arrowheads point to AGT and MLN; their kidney expression was confirmed by RNAscope (Fig. 1h–j). h Endothelial cells are arranged by tissue of origin. e Scatter plots show gene expression levels along the arteriole-to-venule trajectory for the endothelial markers and hormonal genes that are differentially expressed. Shown here is representative data from L4, with similar results in other animals. PCT proximal convoluted tubule, PT proximal tubule, PST proximal straight tubule, LHd loop of Henle thin descending limb, LHa thin loop of Henle thin ascending limb, LHa thick loop of Henle thick ascending limb, DCT distal convoluted tubule, MD macula densa, CDp collecting duct principal cell, Cdiα collecting duct α intercalated cell, CDiβ collecting duct β intercalated cell, VRD vasa recta descending section, VRA vasa recta ascending section.
