Fig. 3: smFRET experiments show consequences of the absence of the NCCA/specifier interactions.
From: Translational T-box riboswitches bind tRNA by modulating conformational flexibility
a Schematic diagram illustrating the smFRET experiment with the tRNAIle-ΔNCCA-Cy5 construct. Cartoon based on a similar cartoon from19. b FRET efficiency histogram for tRNAIle-ΔNCCA-Cy5 mutant. Fitted populations using consensus HMM modeling are shown as in Fig. 2c. c Transition density plot for tRNAIle-ΔNCCA-Cy5 mutant for all data constructed using the idealized Viterbi paths modelled with tMAVEN26, plotted the same way as described in Fig. 2d. d, e Dwell time survival plots for the 0.5 FRET state. The data were fitted using either a single (left) or double (right) exponential decay function. The double exponential decay function better fits the data. Data and fitting results are presented as described in Fig. 2e. f Schematic diagram illustrating the smFRET experiment with the Mtb-ileS T-box riboswitch Δ-Discriminator Mutant and tRNAIle. Cartoon based on a similar cartoon from19. g Representative Cy3 (green) and Cy5 (red) fluorescence intensity (top) and smFRET trajectories (blue, bottom) of the Δ-Discriminator Mutant and tRNAIle complex. h FRET efficiency histogram of the data with the Δ-Discriminator Mutant. Fitted populations using consensus HMM modeling are plotted as in Fig. 2c. i Transition density plot constructed using the idealized Viterbi paths, as described in Fig. 2d. j Dwell time survival plot and fitting results using a single exponential decay function. Data and fitting results are presented as described in Fig. 2e. N in each plot reports the number of traces or events included in the analysis. Source data are provided as a Source Data file.
