Fig. 4: smFRET experiments with tRNAIle-ΔNCCA reveal conformational sampling in the partially bound state.
From: Translational T-box riboswitches bind tRNA by modulating conformational flexibility
a Representative Cy3 (green) and Cy5 (red) fluorescence intensity (top) and smFRET trajectories (blue, bottom) for the One-state population of the Mtb-ileS T-box riboswitch/ tRNAIle-ΔNCCA-Cy5 complex. b FRET efficiency histogram of the One-state population, shown as in Fig. 2c. c Transition density plot for the One-state population constructed using the idealized Viterbi paths, plotted the same way as described in Fig. 2d. d Dwell time survival plots for the 0.5 state in the One-state population, fitted using a single exponential decay function. Data and fitting results are presented as described in Fig. 2e. e Representative fluorescence intensity and smFRET trajectory for the Two-state population of the Mtb-ileS T-box riboswitch/ tRNAIle-ΔNCCA-Cy5 complex. A zoom of a region framed by cyan boxes is displayed on the right, which shows rapid transitions between the 0.5 and 0.7 states. f FRET efficiency histogram of the Two-state population, shown as in Fig. 2c. g Transition density plot constructed using the idealized Viterbi paths for the Two-state population, plotted the same way as described in Fig. 2d. h Dwell time survival plots for the 0.5 and 0.7 states fitted a single exponential decay function. Data and fitting results are presented as described in Fig. 2e. N in each plot reports the number of traces or events included in the analysis. Source data are provided as a Source Data file.
