Fig. 2: Transcriptomic analysis of MG01 before and after engraftment. | Nature Communications

Fig. 2: Transcriptomic analysis of MG01 before and after engraftment.

From: Ready-to-use iPSC-derived microglia progenitors for the treatment of CNS disease in mouse models of neuropathic mucopolysaccharidoses

Fig. 2: Transcriptomic analysis of MG01 before and after engraftment.The alternative text for this image may have been generated using AI.

A UMAP plots of the Bian et al., 2020 dataset of fetal hematopoietic (CD45+) cells depicting the origin, Carnegie stage and cell type grouping of all cells. B Same UMAP plot as (A) displaying expression level of representative markers for Mac 1-3 and Mac 4 groups. C PCA of MG01 (Line 82) and Bian et al. dataset (N = 3 MG01 differentiations). PC plots on the right display the identity score for each cell when compared to the Mac 1-3 or Mac 4 signature. Violin plots display the distribution of results for each sample. D Heatmap of differentially expressed markers in each cell group of Bian et al. when compared to all other groups combined. E Schematic depicting the workflow for bulk RNAseq of engrafted MG01. F Dot plot displaying the average expression and percentage of post-thaw MG01 cells expressing Mac 4 and Mac 1-3 genes in each group. G Heatmap of differentially expressed genes between MG01 and engrafted MG01, including genes in the Mac 4 cell group in D, F (N = 1 MG01 differentiation, 3 technical replicates). Transcript data is scaled by gene. Green arrows highlight known microglial markers. Panel E was in part created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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