Fig. 2: Generation of uniform kidney organoids in UniMat. | Nature Communications

Fig. 2: Generation of uniform kidney organoids in UniMat.

From: Scalable production of uniform and mature organoids in a 3D geometrically-engineered permeable membrane

Fig. 2: Generation of uniform kidney organoids in UniMat.The alternative text for this image may have been generated using AI.

a Confocal z-stack images of kidney organoids cultured in hydrogel layer (Geltrex), UniMat400, UniMat600, and UniMat800, stained with PODXL, LTL, and CDH1. Scale bar = 200 µm. b Measured diameters of kidney organoids formed in hydrogel layer, UniMat400, UniMat600, and UniMat800 (n = 15 organoids, mean ± SD). Significance by one-way ANOVA with Tukey’s multiple comparisons test: PUniMat400, UniMat600 = 1.3 × 10−11, PUniMat600, UniMat800 = 8.5 × 10−5. c Confocal z-stack images for markers of podocytes (PODXL), proximal tubules (LTL), and distal tubules (CDH1), and corresponding Imaris 3D surface rendering images of kidney organoids cultured in hydrogel layer, UniMat400, UniMat600, and UniMat800. Scale bars = 100 µm. d Quantitative analysis of PODXL+ podocytes, LTL+ proximal tubules, and CDH1+ distal tubules fractions with respect to total volume in individual kidney organoids generated in hydrogel layer, UniMat400, UniMat600, and UniMat800 (n = 15 organoids, mean ± SD). Significance by one-way ANOVA with Tukey’s multiple comparisons test. e qRT-PCR of PODXL, SLC34A1, and CDH1 expressions of kidney organoids cultured in hydrogel layer and UniMat (n = 5 independent experiments, mean ± SD). f Organoid formation efficiency of kidney organoids in hydrogel layer and UniMat cultured from three independent batches of differentiation (n = 5 samples, mean ± SD). Significance by one-way ANOVA with Tukey’s multiple comparisons test: Pbatch1, batch2 = 0.0161, Pbatch2, batch3 = 0.0022 (hydrogel layer). Source data are provided as a Source Data file.

Back to article page