Fig. 5: Compared with MCOC1 structure, nucleic acids replace the M1249L C-tail in the EC structure.
From: Structural basis of RNA polymerase complexes in African swine fever virus
a The overall structures of the ASFV RNAP EC and MCOC1 are depicted in cartoon representation. The subunits colors are identical to those in Fig. 2. Helices are represented as cylinders. The nucleic acids are shown in blue (template strand DNA), sky blue (non-template strand DNA), and purple (RNA). Proteins, except for the M1249L C-tail and vRPB6 N-tail in the MCOC1 structure, are shown transparently. b The active center in the EC structure is occupied by the M1249L C-tail. The ASFV RNAP EC and MCOC1 structures are superposed. The nucleic acids in the EC structure, the M1249L C-tail and vRPB6 N-tail in the MCOC1 structure (transparent) are depicted as sticks. c The RNA polymerization activities before and after anti-M1249L antibody purification were assessed by measuring the incorporation of [α−32P]-ATP and normalized against the negative controls lacking RNA polymerase. Each data point represents the mean value of three independent experiments, with error bars indicating standard deviation. Source data are provided as a Source Data file. All calculations were performed using Graphpad Prism 8 software.
