Fig. 2: Hepatic Kmt5c deficiency reduces hepatic glucose output by inhibiting gluconeogenesis.

a Blood glucose levels were measured from 2-month-old (left) (n = 7 each group, female) and 11-month-old (right) (n = 7 each group, male) WT and KO mice under feeding or 24-hour fasting condition. b Western blot analysis (left) and protein quantification (right) of indicated proteins in the livers of 12-week-old male WT and KO mice after 24 h fasting (n = 5 each group). c, d Pyruvate tolerance test was performed in 2-month-old (c) (n = 7 each group, female) and 11-month-old mice (d) (n = 7 each group, male). e, f Glucagon tolerance test was performed in mice of (c) and (d) (n = 7 each group, female for c, male for d). g Glycerol tolerance test was performed in 4-month-old WT and KO mice (n = 6 each group, male). h–j Serum insulin (h) (n = 7 each group), glucagon (i) (n = 7 each group), and corticosterone levels (j) (n = 5 each group) in 12-week-old male WT and KO mice under fed and 24 h fasting conditions. a–j data present mean ± s.e.m; n shown here presents biologically independent animals. a–j two-tailed unpaired Student’s t test. All experiments here were repeated independently three times with similar results. Source data are provided as a Source Data file.