Fig. 5: IORs were decreased in Rab27aash/ash mice. | Nature Communications

Fig. 5: IORs were decreased in Rab27aash/ash mice.

From: In situ architecture of the intercellular organelle reservoir between epididymal epithelial cells by volume electron microscopy

Fig. 5: IORs were decreased in Rab27aash/ash mice.The alternative text for this image may have been generated using AI.

a Representative images of the localization of LAMP1 (magenta) in IORs labeled with Ecad (green) in the control and Rab27aash/ash caput epididymides. White arrowheads: IORs. Lu luminal part. Scale bar: 5 μm. b Percentage of IORs in a single tubule from confocal images in (a) (n = 8 for control, n = 7 for Rab27aash/ash), ****p < 0.0001. c IOR numbers between neighboring PCs (n = 16 cell-pair for control, n = 11 cell-pair for Rab27aash/ash). ****p < 0.0001. Comparison of the IOR area (d) and frequency distribution of the IOR area (e‒g) (n = 74 IORs for control, n = 35 IORs for Rab27aash/ash; 6 fields in each group). ****p < 0.0001. h Quantification of IORs with positive LAMP1 signaling in control and Rab27aash/ash caput epididymides (n = 6 fields in each group). **p < 0.01. i Quantification of intracellular LAMP1 in control and Rab27aash/ash caput PCs (n = 19 cells from 6 fields for each group). ***p < 0.001. Data are presented as mean value ± SEM. Statistical significance between two groups was determined via two-tailed unpaired t-test. Exact p values are provided in Source Data. The source data is provided as a source data file.

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