Fig. 5: Treg loss-of-function experiments reflect their impact in lowering diet-induced hypothalamic immune activation. | Nature Communications

Fig. 5: Treg loss-of-function experiments reflect their impact in lowering diet-induced hypothalamic immune activation.

From: Regulatory T cells in the mouse hypothalamus control immune activation and ameliorate metabolic impairments in high-calorie environments

Fig. 5: Treg loss-of-function experiments reflect their impact in lowering diet-induced hypothalamic immune activation.The alternative text for this image may have been generated using AI.

a–f Analyses of frequencies of CD80+, CD86+, and MHCII+ cells in CD45intCD11b+ microglia of a–c C57Bl/6J or d–f Balb/c mice on standard diet examined 2 weeks after Treg depletion using mCD25 antibodies. Depicted are Box-and-whisker plots (min-to-max values with single data points). n = 6 biological replicates from two independent experiments. Two-tailed unpaired t test, P(a) = 0.1054; P(b) = 0.0499; P(c) = 0.0491; P(d) = 0.0292; P(e) = 0.3581; P(f)= 0.3513. g–i Identification of frequencies of CD80+, CD86+, and MHCII+ cells in CD45hiCD11b+ macrophages of C57Bl/6J mice on standard diet examined 2 weeks after Treg depletion using mCD25 antibodies. Depicted are Box-and-whisker plots (min-to-max values with single data points). Two-tailed unpaired t test, P(g) = 0.4956; P(h) = 0.0013; P(i) = 0.0326. Source data are provided as a Source Data file. *P < 0.05. **P < 0.01.

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