Fig. 5: The degradation of protein H2B. | Nature Communications

Fig. 5: The degradation of protein H2B.

From: In situ construction of intracellular supramolecular assemblies as an alternative strategy for protein degradation

Fig. 5: The degradation of protein H2B.The alternative text for this image may have been generated using AI.

a, b Western blotting showing that (a) the degradation of H2B protein in HeLa cells depends on TP2T concentration, but (b) no degradation of H2B protein in HUVECs. c Quantitation of the corresponding relative protein expression levels (above: HeLa cells, below: HUVECs). d, e Western blotting showing that (d) the degradation of H2B in HeLa cells depends on Tz-MitoFlag concentration, but (e) no degradation of H2B in HUVECs. f Quantitation of the corresponding relative protein expression levels (above: HeLa cells, below: HUVECs). g Mechanistic investigation of H2B degradation. h, i Western blotting to determine the mechanism of H2B protein degradation (h) with or without inhibitors and (i) with or without the in situ formation of SA-PROTAC assemblies (n  =  3 independent experiments, and the values are presented as the mean ± S.E.M.). One-way analysis of variance (ANOVA) was performed, and Tukey’s test was used to correct for multiple comparisons via a statistical hypothesis. The immunoblotting experiments were repeated three times with similar results (h, i). Source data are provided as a Source Data file.

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